DNA Polimerasa 1U/µl

DNA polimerasa recombinante de Thermus sp. y clonada en E. coli, con actividades de polimerasa 5'-3' y de exonucleasa 5'-3' y una tasa de error reducida (1 - 10x10^-6). Enzima termoestable, perfecta para amplificaciones rutinarias incluyendo:

• Amplificación de hasta 5 productos de PCR
• Cribado de colonias 
• Construcción de ADNc recombinante
• Genotipado 

Contenido:

Biotools DNA Polymerase (1U/µl y 5U/µl) se proporciona, según la elección del cliente, con 10X Standard Reaction Buffer con 2mM MgCl₂ ó con tampón de reacción sin MgCl₂ (10X Reaction Buffer MgCl₂ Free) más un tubo separado de MgCl₂ para optimizar la concentración de magnesio.

Haz una pregunta sobre DNA Polimerasa>>

• BOLETÍN DNA POLYMERASE 1U  >>    
• MSDS DNA POLYMERASE  >> 

FLYERS

• Nidda Syeed et al., A Case-Control Study of TP53 R72P Polymorphism in the Breast Cancer Patients of Ethnic Kashmiri Population. World J Oncol. 1(6):236-241 (2010)
• Saija J. Ahonen et al., A CNGB1 Frameshift Mutation in Papillon and Phalene Dogs with Progressive Retinal Atrophy. PLoS ONE , Volume 8, Issue 8, e72122 (August 2013)
• K. Rikalainen et al., A large panel of novel microsatellite markers for the bank vole (Myodes glareolus). Molecular Ecology Resources  8, 1164–1168 (2008)
• I. Doadrio el al., A new species of the genus Saqulius bonaparte, 1837 from the Tagus river basin. Graellsia, 63(1): 89-100 (2007)
• S. Kami?ski et al., A note on associations between polymorphism within the 2,4-dienoyl-CoA reductase gene (DECR1) and growth rate of Polish Landrace boars.Journal of Animal and Feed Sciences, 18, 71–77 (2009)
• Ana Isabel Camacho et al., A note on the systematic of the Bathynellacea (Crustacea, Malacostraca) using molecular evidence. Contributions to Zoology, 71 (4), Vol. 82.nr.2 (2002)
• Kaisa Kyö stilä et al., A SEL1L Mutation Links a Canine Progressive Early-Onset Cerebellar Ataxia to the Endoplasmic Reticulum–Associated Protein Degradation (ERAD) Machinery. PLoS Genetics,  Volume 8, Issue 6,| e1002759 (June 2012)
• María A. Balboa et al., Amplification Mechanisms of Inflammation: Paracrine Stimulation of Arachidonic Acid Mobilization by Secreted Phospholipase A2 Is Regulated by Cytosolic Phospholipase A2-Derived Hydroperoxyeicosatetraenoic Acid. The Journal of Immunology, 171: 989–994 (2003)
• EM Klumb et al., Are women with lupus at higher risk of HPV infection? Lupus  19, 1485–1491 (2010)
• Arshad A. Pandithet al., Association of Chemokine and Chemokine Receptor Gene Polymorphis (MCP1 A-2518G and CCR2-V64I) with Urinary Bladder Cancer: A Study in Kashmiri Population. American Journal of Molecular and Cellular Biology Vol. 2 No. 1 pp. 1-13 (2013)
• S. Kimi Uehara and G. Rosa, Association of uricemia with biochemical and dietary factors in human adults with metabolic syndrome genotyped to C677T polymorphism in the methylenetetrahydrofolate reductase gene. Nutr Hosp. 26(2):298-303 (2011)
• Maayan Amit et al., Biased exonization of transposed elements in duplicated genes: A lesson from the TIF-IA gene. BMC Molecular Biology, 8:109 (2007)
• J. F. Siqueira Jr,  I. N. Rôças, Campylobacter gracilis and Campylobacter rectus in primary endodontic infections. International Endodontic Journal, 36, 174:180, (2003)
• Sueli M. Oba-Shinjo et al., Cancer-testis (CT) antigen expression in medulloblastoma. Cancer Immunity Vol. 8, p. 7 (22 April 2008)
• Eva M. Martínez-Conesa et al., Characterization of ocular surface epithelial and progenitor cell markers in human adipose stromal cells derived from lipoaspirates. IOVS Papers in Press. As Manuscript iovs.11-7550. Published on December 22, (2011)
• Amalia Fernández-Martínez et al., Characterization of the Plasmodium falciparum Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase Gene in Samples from Equatorial Guinea before Implementation of Artemisinin-Based Combination Therapy. Am. J. Trop. Med. Hyg., 88(1), pp. 43–47 (2013)
• Amalia Fernández-Martínez et al., Characterization of the Plasmodium falciparum Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase Gene in Samples from Equatorial Guinea before Implementation of Artemisinin-Based Combination Therapy. Am. J. Trop. Med. Hyg., 88(1), pp. 43–47 (2013)
• Alberto Vicens, Eduardo R. S. Roldan, Coevolution of Positively Selected IZUMO1 and CD9 in Rodents: Evidence of Interaction Between Gamete Fusion Proteins? BOR Papers in Press. DOI:10.1095/biolreprod.113.116871, Published on March 26, (2014)
• Rosa A García-Fernández et al., Combined loss of p21waf1/cip1 and p27kip1 enhances tumorigenesis in mice. Laboratory Investigation 91, 1634–1642 (2011) 
• Cristina Moldes et al., Construction of a Chimeric Thermostable Pyrophosphatase To Facilitate Its Purification and Immobilization by Using the Choline-Binding Tag. Applied and Environmental Microbiology, Vol.70 nº 8, p. 4642–4647 (Aug. 2004)
• Ana Crespo et al., Coscinocladium, an overlooked endemic and monotypic Mediterranean lichen genus of Physciaceae, reinstated by molecular phylogenetic analysis. Taxon 53 (2), 405-414 (May 2004)
• Elena García-Vigón et al., Cross-ampliffied Polymorphic Microsatellites for the spotless starling sturnus unicolor. Ardeola 55(1), 3-11 (2008)
• Guadalupe Miró et al., Current status of L. infantum infection in stray cats in the Madrid region (Spain): implications for the recent outbreak of human leishmaniosis?Miró et al. Parasites & Vectors , 7:112 (2014)
• I. Martín et al., Detection of cat, dog, and rat or mouse tissues in food and animal feed using species-specific polymerase chain reaction. J ANIM SCI, 85:2734-2739 (2007)
• I. Martín et al., Detection of chicken, turkey, duck, and goose tissues in feedstuffs using species-specific polymerase chain reaction. J ANIM SCI, 85:452-458 (2007)
• Bruhvi Poptani et al., Detection of Enterococcus faecalis and Candida albicans in previously root-filled teeth in the population of Gurajat with polymerase chain reaction. Contemporary Clinical Dentistry, J, Vol 4, Issue 1 (an-Mar 2013)
• Miriam Pape et al., Differential Expression of the Chlamydomonas [FeFe]-Hydrogenase Encoding HYDA1 Gene Is Regulated by the COPPER RESPONSE REGULATOR. Plant Physiology, Vol. 159, pp. 1700–1712 (August 2012)
• N Prat et al., Distribution, Abundance and Molecular Analysis of Genus Barbadocladius Cranston & Krosch (Diptera, Chironomidae) in Tropical, High Altitude Andean Streams and Rivers. Neotrop Entomol DOI 10.1007/s13744-013-0161-1, (2013)
• Svetlana Solovieva et al., Vitamin D receptor gene polymorphisms and susceptibility of hand osteoarthritis in Finnish women. Arthritis Research & Therapy, 8:R20  (2006)
• Leonor C. Bedoya et al., Visual Tracking of Plant Virus Infection and Movement Using a Reporter MYB Transcription Factor That Activates Anthocyanin Biosynthesis. Plant Physiology, Vol. 158, pp. 1130–1138 (March 2012)
• Celia Perales et al., Enhancement of DNA, cDNA synthesis and ®delity at high temperatures by a dimeric single-stranded DNA-binding protein. Nucleic Acids Research, Vol. 31, No. 22 6473±6480 (2003)
• Heidi M.T. Kunttu et al., Flavobacterium columnare colony types: Connection to adhesion and virulence? Microbial Pathogenesis 46, 21–27 (2009) 
• Barbara Pipan et al., Genetic differentation among sexsually compatible relatives of Brassica napus L. Genetika, Vol. 45, No.2,309-327, (2013)
• D.C. Salim et al., Genetic variability in maned wolf based on heterologous short-tandem repeat markers from domestic dog. Genet. Mol. Res. 6 (2): 348-357 (2007)
• K. C. Park et al., Genetic variations of AA genome Oryza species measured by MITE-AFLP. Theor Appl Genet 107:203–209 (2003)
• High dispersal potential has maintained long-term population stability in the North Atlantic copepod Calanus finmarchicus. Proc. R. Soc. B 276, 301–307 (2009)
• Nasir Mohamad et al., High Frequency of CYP3A4*1B among Opiate Dependent Patients in Malaysia. J Addict Res Ther, Volume 3, Issue 3, 1000130 (2012)
• Renata Moreno et al., High-Level Overproduction of His-Tagged Tth DNA Polymerase in Thermus thermophilus. Applied and Environmental Microbiology, Vol.71 nº 1, p. 591–593 (Jan. 2005)
• Daniel Wysokinski et al., Variability of the Transferrin Receptor 2 Gene in AMD. Hindawi Publishing Corporation Disease Markers Volume, Article ID 507356, 8 pages (2014)
• Wen-Ji Yeh et al., Identification of a Blue-Green Alga Arthrospira maxima Using Internal Transcribed Spacer Gene Sequence. AOT, Vol. 42, No. 1,25-37, (Dectember 2004)
• Lucía Fernández et al., The iron- and temperature-regulated haemolysin YhlA is a virulence factor of Yersinia ruckeri. Microbiology 153, 483–489  (2007)
• G. O. Zoletti et al., Identification of Enterococcus faecalis in Root-filled Teeth With or Without Periradicular Lesions by Culturedependent and Independent Approaches. JOE, Volume 32, Number 8, (August 2006)
• Pirjo Tanhuanpää et al., The first doubled haploid linkage map for cultivated oat. Genome 51: 560-569 (2008)
• Esther Jiménez et al., Is meconium from healthy newborns actually sterile? Research in Microbiology 159, 187-193 (2008)
• Palloma Rodrigues Marinho et al., Marine Pseudomonas putida: a potential source of antimicrobial substances against antibiotic-resistant bacteria. Mem Inst Oswaldo Cruz, Vol. 104(5): 678-682, (August 2009) 
• Sueli M. Oba-Shinjo et al., Melanocyte Transformation Associated with Substrate Adhesion Impediment. Neoplasia, Vol. 8, No. 3, pp. 231 – 241 (March 2006)
• Heikki T Turunen et al., Members of the murine Pate family are predominantly expressed in the epididymis in a segment-specific fashion and regulated by androgens and other testicular factors. Reproductive Biology and Endocrinology, 9:128 (2011)
• Terhonen, E et al., The Effect of Latitude, Season and Needle-Age on the Mycota of Scots Pine (Pinus sylvestris) in Finland. Silva Fennica 45(3): 301–317 (2011)
• María Isabel Martínez-Nieto et al., Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae. Int. J. Mol. Sci. 13, 11861-11869 (2012)
• Carmen Mansilla et al., The diagnosis of the tomato variant of pepino mosaic virus: An IC-RT-PCR approach. European Journal of Plant Pathology 109: 139–146, (2003)
• Cristina Adán et al., Mitochondrial Transcription Factor B2 Is Essential for Metabolic Function in Drosophila melanogaster Development. The Journal of Biological Chemistry,  Vol. 283, No. 18, pp. 12333–12342, (May 2, 2008) 
• J C Sánchez-Gutiérrez et al., Molecular analysis of the aldolase B gene in patients with hereditary fructose intolerance from Spain. J Med Genet; 39:e56 (2002)
• Oscar Blanco et al., Molecular phylogeny of parmotremoid lichens (Ascomycota, Parmeliaceae). J Med Genet;39:e56. Mycologia, 97(1), pp. 150–159.  (2005)
• Patricia Martorell et al., Molecular typing of the yeast species Dekkera bruxellensis and Pichia guilliermondii recovered from wine related sources. International Journal of Food Microbiology 106, 79 – 84 (2006)
• Domínguez-Domínguez Omar et al., Morphological and genetic comparative analyses of populations of Zoogoneticus quitzeoensis (Cyprinodontiformes:Goodeidae) from Central Mexico, with description of a new species. Revista Mexicana de Biodiversidad 79: 373- 383, (2008)
• Neetu Singh et al., Morphological redescription and molecular characterization of three species of Travassosinematidae (Nematoda: Oxyurida: Thelastomatoidea) from Gryllotalpa africana Beauv (Orthoptera: Gryllotalpidae). Bioinformation 9(16): 818-823 (2013)
• L Pérez-Pardal et al., Multiple paternal origins of domestic cattle revealed by Y-specific interspersed multilocus microsatellites. 1–9, Heredity (2010) 
• Carolina Marín, Multiplex-polymerase chain reaction assay for the detection of prognostically significant translocations in acute lymphoblastic leukemia. Haematologica; 86:1254-1260 (2001)
• Sll Hoe et al., Mutational analysis of p53 and RB2/p130 genes in Malaysian
  nasopharyngeal carcinoma samples: a preliminary report. Malaysian J Pathol; 28 : 35 – 39 (2006)
• Thuy-Huong Ta Tang et al., Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region. Mem Inst Oswaldo Cruz, Vol. 105(6): 823-828, (September 2010)
• Valéria Maria Lara et al., Occurrence of feline immunodeficiency virus infection in cats. Ciência Rural, Santa Maria, v. 38, n. 8, p. 2245-2249 (nov, 2008)
• Mariano Bulos et al., Occurrence of the rust resistance gene Lr37 from Aegilops ventricosa in Argentine cultivars of wheat. Electronic Journal of Biotechnology, Vol.9 No.5 (Issue of October 15, 2006)
• Luciana da Silva Ruiza et al., Outbreak of fungemia caused by Candida parapsilosis in a neonatal intensive care unit: Molecular investigation through microsatellite analysis. Rev Iberoam Micol. 30(2):112–115 (2013)
• I. Martín et al., Technical note: Detection of chicken, turkey, duck, and goose tissues in feedstuffs. J ANIM SCI, 85:452-458 (2007)
• Ahmed. N. Sharaf et al., Overexpression of rice chitinase gene: Evaluation of chitinase ability as a bio-antifungal agent. Arab J. Biotech., Vol. 12, No. (1) :85-98 (Jan. 2009)
• Michael R. Ziebell et al., Peptides that mimic glycosaminoglycans: high-affinity ligands for a hyaluronan binding domain. hemistry & Biology 8  1081-1094 (2001)
• Hemlata Kumari Das, Phylogenetic Relationship of Wild and Cultivated Oryza species using SSR Markers. Int J Biol Technology:4(3):23-31 (2013)
• Nadia Haider et al., Phylogenetic relationships among date palm (Phoenix dactylifera L.) cultivars in Syria using RAPD and ISSR markers. Journal of Plant Biology Research, 1(2): 12-24 (2012)
• Martín I. et al., Polymerase chain reaction Detection of rabbit DNA in food and animal feed. World Rabbit Sci. 17: 27 - 34 (2009)
• José F. Siqueira,  Isabela N. Rôças, Polymerase chain reaction–based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod;97:85-94 (2004)
• Gregory Leon, Paul H. Roy, Potential Role of Group IIC-attC Introns in Integron Cassette Formation. Vol. 191, No. 19, p. 6040–6051 (Oct 2009)
• A. Almodóvar et al., Preliminary results from characterization of the Iberian Peninsula sturgeon based on analysis of the mtDNA cytochrome b. Bol. Inst. Esp. Oceanogr. 16 (1-4). pp: 17-27 (2000)
• Yilma Çakaloglu et al., Prevalence and clinical significance of SEN-H virus in chronic hepatitis B, C and delta infections in Turkey. The Turkish journal of Gastroenterology, Volume 19, No 2, Pages 104-108 (2008)
• Annie Machordom et al., Rapid radiation and cryptic speciation in squat lobsters of the genus
  Munida (Crustacea, Decapoda) and related genera in the South West Pacific: molecular and morphological evidence. Molecular Phylogenetics and Evolution 33 259–279 (2004)
• Francisco López-Martínez et al., Role of MYOC and OPTN sequence variations in Spanish patients with Primary Open-Angle Glaucoma. Molecular Vision; 13:862-72 (2007)
• Alicia Barroso-delJesus et al., Selection of targets and the most efficient hairpin ribozymes for inactivation of mRNAs using a self-cleaving RNA library. EMBO reports vol. 2, no. 12, pp 1112–1118 (2001)
• Noelia López-Sánchez et al., Single mage gene in the chicken genome encodes CMage, a protein with functional similarities to mammalian type II Mage proteins. Physiol Genomics 30: 156–171 (2007)
• Maite Buxens et al., Substitution of Nitrite Reductase of Thermosynechococcus elongatus BP-1 by the Homologous Gene of Phormidium laminosum. Advances in Microbiology, 3, 69-79 (2013)
• P. Tantasawat et al., SSR analysis of soybean (Glycine max (L.) Merr.) genetic relationship and variety identification in Thailand. AJCS 5(3):283-290 (2011)
• Moreira, B. et al., Genetic component of flammability variation in a Mediterranean shrub. Molecular Ecology, 23:1213-1223 (2014)