High Scriptools-Quantimix Easy Probes Kit

High Scriptools-Quantimix Easy Probes Kit is a novel real-time RT-PCR system for the quantification of RNA targets in an easy-to-handle format. The kit has been designed to use with hydrolysis probes (e.g. TaqMan probes). The high specificity and sensitivity of this kit is achieved by the use of the new High SQP Retrotrascriptase and the Biotools HotSplit DNA Polymerase, together with a specialised buffer.

The kit has been designed to deliver maximum efficiency, precision and sensitivity of quantitative RT-PCR. For these purpose two high-performance enzymes, a thermostable reverse transcriptase and a DNA polymerase with hot start activity, carry out reactions. Both cDNA synthesis and PCR are performed sequentially in a single tube due to a single buffer system formulated to ensure specific primer annealing.

High Scriptools-Quantimix Easy Probes Kit provides a convenient and fast procedure, one step only, for synthesizing cDNA and DNA amplification in real time. All reagents necessary for both reactions are added in one tube at the same time which confers an easy-to-handle format saving manipulation time and reducing contamination risks without compromising the efficiency or sensitivity of the kit.

The kit has been optimised with RNA from different origins including RNA virus (e.g. RNA from HCV; HIV; and H1N1 virus, among others).

REAGENTS INCLUDED IN THE KIT

The system contains sufficient reagent for a number of one-tube RT-PCR reactions of 50µl.

• High SQP Master Mix: An easy and convenient 2X Master Mix formulated to facilitate both efficient reverse transcription and specific amplification in a one-tube format. OneStep RT-PCR buffer system and has been specifically adapted for quantitative RT-PCR analysis using sequence-specific probes. The mixture includes: Biotools HotSplit DNA Polymerase; dNTPs; MgSO_4; and Reaction Buffer.
• High SQP Retrotranscriptase: A new RNase H minus, thermostable reverse transcriptase which exhibits high affinity for RNA and works in a higher range of temperature than most commercially retrotranscriptases. The RT enzyme is provided in a separate tube.
• 100 mM MgSO₄ Solution: Used only for assays which require an additional optimisation.

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