HIGH RNase INHIBITOR

High RNase Inhibitor inhibits, by binding in a potent non-competitive inhibitor of a wide spectrum of RNases, including RNase A, RNase B and RNase C. It is a recombinant mammalian protein, manufactures in E. coli, with a monomeric structure of 49.6 kDa.

Applications

The inhibitor protects RNA in the following applications:

• First strand cDNA synthesis
• RNA amplification
• RNA purification and storage
• In vitro transcription
• In vitro translation
• Separation and identification of specific ribonuclease activities
• Removal of gDNA from RNA preparations
• Synthesis of RNA probes
• Synthesis of cDNA probes 
Storage conditions 
High RNase Inhibitor can be store at - 20°C in a constant temperature freezer through the expiration date printed on the label. 
Avoid exposure to frequent temperature changes. For frequent use divide the product in aliquots to avoid multiple freeze-thaw cycles.

Activity Unit

One unit of the High RNase Inhibitor inhibits 5 ng of RNase A by 50%.

Activation-inactivation

The High RNase Inhibitor protects RNA from degradation at temperatures ≤ 55 °C.

This product is inactivated by heating at 75 °C for 10 minutes. Common denaturants and oxidizing (eg. SDS, urea) strongly inhibit the High RNase Inhibitor and release the RNase bound.

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