DNA Amplitools HotSplit Master Mix

The new developed DNA AmpliTools HotSplit Master Mix (2X) is an optimised ready-to-use solution containing HotSplit DNA Polymerase for performing PCR amplifications of a wide range of templates (including genomic DNAs and templates rich in CG-content). It is recommended for amplification up to 10 kb in length.

The hot start nature of the HotSplit DNA Polymerase offers convenience with decreased interference from primer dimers and secondary products. The Biotools DNA AmpliTools HotSplit Master Mix makes highly specific hot-start PCR extremely simple with minimal optimisation required. PCR setup is quick and convenient as all reaction components can be combined at room temperature.

It is a ready-to-use 2X master mix that reduces set up time, liquid handling steps and prevents cross-contamination. With the exception of primers and template, the DNA AmpliTools HotSplit Master Mix provides all the necessary reagents at optimal concentrations to perform PCRs. The DNA AmpliTools HotSplit Master Mix (2X) works with different reaction volumes between 20-25 μl. The DNA AmpliTools Master Mixes of Biotools allow for convenient and easy to use PCRs, and they are ideally suited to routine PCR applications.

Applications of DNA AmpliTools HotSplit Master Mix:

• High specificity PCR (up to 10 kb)
• Routine PCR
• High throughput PCR
• Primer extension
• Generation of PCR products for TA cloning
• Gene sequencing
• RT-PCR

Note: This master mix is not recommended for certain experiments dealing with amplification of
sequences homologous to those found in E. coli.

Components: 
The DNA AmpliTools HotSplit Master Mix (2X) contains recombinant Biotools HotSplit DNA Polymerase; dNTPs; MgCl₂; Reaction Buffer; and stabilisers at optimal concentrations for efficient DNA amplifications.

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