DNA AmpliGel Master Mix-Plates

Biotools has developed a new PCR product using one of its international patents: GELIFICATION. The process is simple because all the reagents needed for the PCR reaction are included in each vial, so just add the sample (DNA and primers). 

Features:

READY-TO-USE 

• One well- One reaction format
• Ready for in-field manipulation
• No optimization needed

LESS MANIPULATION & LESS CONTAMINATION

• Only need to add primers and DNA sample.
• Customized plates available: Give us your primers and we will include them in the Gelified reaction.

SHIPMENT AT ROOM TEMPERATURE AND STORAGE AT 4ºC

• Stable for long time periods at 4ºC.

HOT-START EFFECT

• Reaction starts when the Gel is completely rehydrated with the increase of temperature.

NO NEED TO RESUSPEND

• Reaction will become liquid automatically with the increase of temperature (no handling).

ADVANTAGES vs LYOPHILIZATION

• Components keep their activity.
• No need to resuspend. 
• Final concentration is mantained.

DNA AmpliGel Master Mix - Plates and the gelified mix reagents can be stored at 4 °C respectively for long periods of time. The Ready-to-Use format minimises risk of contaminations, while promoting rapid sample processing ideal for routine applications. 

This enzyme is a recombinant DNA Polymerase from Thermus sp. and cloned in E. coli, with both 5'-3' polymerase and 5'-3' exonuclease activities, and reduced error rates (1 - 10x 10^-6). 

Thermostable enzyme perfect for routine amplifications including:

• Amplification of PCR products of up to 5 Kb 
• Colony screening 
• Recombinant cDNA construction 
• Genotyping 
• Routine DNA amplifications 

DNA AmpliGel Master Mix - Plates is available in Vials (fv 25 µl) and in 96 well Plates (fv 25 µl). 

Contents:

DNA AmpliGel Master Mix - Plates 96 well Plates is supplied with 10 X Standard Reaction Buffer including 2 mM MgC₂ and dNTPs. 

DNA AmpliGel Master Mix - Plates Vials (0,2 vials) 96 vials. Each vial includes Standard Reaction Buffer with 2 mM MgCl₂, dNTPs, and DNA polymerase.

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FLYERS

• J.K. Purzycka et al., Efficiency comparison of seven different Taq polymerases used in hemogenetics. International Congress Series 1288 (2006) 719– 721
• Victor Parro et al., A Microbial Oasis in the Hypersaline Atacama Subsurface Discovered by a Life Detector Chip: Implications for the Search for Life on Mars, Astrobiology. Volume 11, Number 10, pp: 969-996 (2011)
• Jonas Høgberg et al., Gelification- a simple and efficient method por on-chip storage of reagents: Towardss lab-on-a-chip systems for point-of-care diagnostics, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Seattle, Washington, USA. pp:1864-1866 (Oct-2011)
• Yi Sun et al., Pre-storage of gelified reagents in a lab-on-a-foil system for rapid nucleic acid analysis, Lab on a Chip, 13, pp: 1509–1514 (2013)