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Biotools High Retrotranscriptase-Starter Kit is a complete system for efficient synthesis of first strand cDNA.
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Biotools High Retrotranscriptase-Starter Kit is a complete system for efficient synthesis of first strand cDNA. The kit uses the Biotools High Retrotranscriptase, a new reverse transcriptase that features a high thermostability and lacks the RNase H activity. The enzyme provides first strand cDNA synthesis with a broader optimal reaction temperature from 40°C to 65°C. Due to its thermostability, Biotools High Retrotranscriptase can transcribe GC-rich templates with large amounts of secondary structure without the help of reaction additives.
Biotools High Retrotranscriptase-Starter kit is supplied with both Oligo (dT)15 Primer and Random Primer. Random hexamer primers bind non-specifically and are used to synthesise cDNA from all RNAs in total RNA population. The Oligo (dT)15 Primer selectively anneals to the 3'-end of poly(A) RNA, synthesising cDNA only from poly(A) tailed mRNA. Gene-specific primers may also be used with the kit to prime synthesis from a specified sequence. The kit contains a recombinant RNase Inhibitor, High RNase Inhibitor, which protects RNA from degradation by RNases A, B and C.
The first strand cDNA products generated by Biotools High Retrotranscriptase are suitable for downstream gene cloning, analysis by standard PCR or real-time PCR.
KIT COMPONENTS
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